Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc TRIS-HCL Buffer 1.0M, pH 7.2 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 7.2 (0.2 micron filtered), 500 ml.
Chrono Log Corporation Saline (0.9%), 15mL
This item has a minimum qty of 4 per supplier requirements.
Sterile physiological saline for irrigation (0.85% or 0.9% w/v) for CHRONOPAR Reagent preparation and for dilution of the Whole Blood specimen.
Medchemexpress LLC Beta-hydroxyisovalerylshikonin | 7415-78-3 | 1 MG
Beta-hydroxyisovalerylshikonin is a natural product isolated from *Lithospermum erythrorhizon*. It acts as a potent inhibitor of protein tyrosine kinases (PTK) and is effective against a wide variety of tumor cell lines.
- Potent inhibitor of protein tyrosine kinases (PTK)
- Effective against various tumor cell lines
- Induces cell death in NCI-H522 and DMS114 cells
- For research use only
Medchemexpress LLC Rasagiline-13C3 mesylate | 1391052-18-8 | 270.32 | 1 MG
Rasagiline-13C3 mesylate is a deuterium-labeled Rasagiline mesylate, which acts as a highly potent, selective, and irreversible mitochondrial monoamine oxidase (MAO) inhibitor. It exhibits IC50s of 4.43 nM and 412 nM for rat brain MAO B and A activity, respectively.
- Used as a tracer.
- Internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.
- Incorporates stable heavy isotopes into drug molecules for quantitation.
- Deuteration can affect the pharmacokinetic and metabolic profiles of drugs.
Medchemexpress LLC MOPS (sodium salt) | 71119-22-7 | 99.8% | 25 G
MOPS sodium salt is a buffering agent utilized in biological applications. MOPS buffer is effective in maintaining the pH of mammalian cell culture media and has been shown to significantly improve the thermal stability of BSA. It is relatively safe for short-term experiments, though it may reduce cell viability after 4 hours, suggesting experimental duration limits of 4-12 hours.
- Buffering agent for biological research.
- Maintains pH in mammalian cell culture media.
- Significantly improves the thermal stability of BSA.
- Relatively safe for short-term experiments, though it may reduce cell viability after 4 hours, suggesting experimental duration limits of 4-12 hours.
- Can be used in the preparation of keratinocyte and microbial culture media.