Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.5) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
National Diagnostics PROTOGEL RESOLVING BUFFER 1 LT
ProtoGel Resolving Buffer (4X), 4X Laemmli Tris-HCl resolving gel buffer for clear, reproducible SDS-PAGE, 1 LT
National Diagnostics TRIS-GLYCINE-SDS PAGE BUFFER (
Tris-Glycine-SDS PAGE Buffer (10X), Ultra-pure concentrated standard running buffer for SDS-PAGE. Formulated with 18 MegOhm water. 0.2 micron filtration, 4 LT
National Diagnostics TBE BUFFER (10X) 4 LT
TBE Buffer (10X), Formulated with 18MegOhm water. 0.2 micron filtration. The most stable TBE on the market, 4 LT
Andwin Scientific TRIS 1M ST SOLUTION PH9 250ML
Tris 1.0 M Sterile Solution, pH 9.0, Ultra Pure Grade, 250ml
Aniara Diagnostica LLC Phosphate Buffered Saline with Tween 20, pH 7.4, 100 tablets
Medicago Phosphate Buffered Saline w/Tween (PBS-T) pH 7.4 supplied as preweighed tablets yields 1000 mL 0.14 M NaCl, 0.0027 M KCl, 0.05% Tween-20, 0.01 M phosphate buffer with pH 7.4 at 25C. Use for immunoassays. Bottle of 100 Tabelts.
Kd Medical Inc STERILE SALINE 0.9%, 100ML BTL
Solution used for sterile irrigation, rinsing, dilution and cell washing purposes. One 100ml bottle.
Sigma Aldrich Fine Chemicals Biosciences HEPES buffered saline BioUltra, for molecular biology, 2x concentrate | MFCD00006158 | 50ML
HEPES buffered saline BioUltra, for molecular biology, 2x concentrate | MFCD00006158 | 50ML
VWR INTERNATIONAL LLC TRIS-Borate-EDTA Buffer, 10X LIQUID CONCENTRATE, Ultra Pure Grade4l
TRIS-Borate-EDTA Buffer, 10X LIQUID CONCENTRATE, Ultra Pure Grade | 4l
Vmrd Inc 4X FA Powdered Rinse Buffer
FA Rinse Buffer, pH 9.0. Powder to make one liter of 4x rinse buffer. Used to rinse fluorescent antibody antibody substrate slides.
Sigma Aldrich Fine Chemicals Biosciences Phosphate-Citrate Buffer tablet | 50TAB
Phosphate-Citrate Buffer tablet | 50TAB
Electron Microscopy Sciences D-PBS, Formula II Phosphate Buffer Saline 1 LT
All of our Dullbecco's Phosphate Buffer Saline Solutions contain Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Solutions are prepared 1X strength.