Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences BICINE | 150-25-4 | MFCD00004295 | 250g
BICINE | Purity: 99% | Mol Wt: 163.17 | 150-25-4 | MFCD00004295 | 250g
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Buffer
Dulbecco Phosphate Buffered Saline (DPBS) is a balanced salt SOLUTIONon.
Sigma Aldrich Fine Chemicals Biosciences Tricine | 5704-04-1 | MFCD00004277 | 100 g
Tricine | Purity: ≥99% | Mol Wt: 179.17 | 5704-04-1 | MFCD00004277 | 100 g
Sigma Aldrich Fine Chemicals Biosciences Tricine | 5704-04-1 | MFCD00004277 | 25g
Tricine | Purity: ≥99% (titration) | MW:179.17 | 5704-04-1 | MFCD00004277 | 25g
Sigma Aldrich Fine Chemicals Biosciences PIPES 99 titration25KG
PIPES is a member of the ethanesulfonic acid buffer series first introduced by Good et al. developed to meet certain criteria midrange pKa maximum water solubility and minimum solubility in all other solvents minimal salt effects minimal change in pKa with temperature chemically and enzymatically stable minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 C is near physiological pH PIPES has applications in cell culture work.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
Sigma Aldrich Fine Chemicals Biosciences HEPES sodium salt solutin500ML
HEPES is a general-purpose zwitterionic buffer which does not bind magnesium calcium manganese(II) or copper(II) ions. HEPES has been classified as Good buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.
Sigma Aldrich Fine Chemicals Biosciences HEPES, 7365-45-9, MFCD00006158, 500g
Empirical Formula (Hill Notation) C8H18N2O4S, ≥99.5% (titration), Molecular Weight 238.30, Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Sigma Aldrich Fine Chemicals Biosciences HEPES 99.5 (titration), 7365-45-9, MFCD00006158
HEPES 99.5 (titration)
Sigma Aldrich Fine Chemicals Biosciences PIPES disodium salt | 76836-02-7 | MFCD00064349 | 100 g
PIPES disodium salt | Purity: ≥99% | Mol Wt: 346.33 | 76836-02-7 | MFCD00064349 | 100 g
Sigma Aldrich Fine Chemicals Biosciences BIS TRIS propane BioXtra 9100G
BIS TRIS propane BioXtra 9100G
Sigma Aldrich Fine Chemicals Biosciences HEPES BioUltra for molecul50G
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Sigma Aldrich Fine Chemicals Biosciences MOPSO sodium salt 99 titra500G
MOPSO sodium salt 99 titra500G
Sigma Aldrich Fine Chemicals Biosciences Hanks Balanced Salts Modif1L
HANKS Hanks Balanced Salts Modif1L
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
DULBECCOS Dulbeccos Modified Eagles1L